Detection of Group B Streptococcal Colonization in Pregnant Women Using Direct Latex Agglutination Testing of Selective Broth
نویسندگان
چکیده
منابع مشابه
Detection of group B streptococcal colonization in pregnant women using direct latex agglutination testing of selective broth.
Group B streptococcus (GBS) is the leading cause of neonatal infection in the United States. In May 1996, the Centers for Disease Control and Prevention (CDC) developed a strategy to reduce the morbidity and mortality of GBS-associated neonatal disease (2). They recommended culturing all pregnant women at 35 to 37 weeks gestation for GBS colonization using vaginal-rectal specimens. For maximum ...
متن کاملComparison of NNA agar culture and selective broth culture for detection of group B streptococcal colonization in women.
In 1996, the Centers for Disease Control and Prevention recommended the use of a selective broth culture for the improved detection of genital tract or anorectal carriage of group B streptococci (GBS) in pregnant women. In order to verify this recommendation in our laboratory, we compared the sensitivity of Todd-Hewitt medium with gentamicin and nalidixic acid (SBM) with our current method of d...
متن کاملDetection of group B streptococci by agglutination testing from selective broth cultures.
A rapid technique for the immunological identification of group B streptococci in vaginal swabs is reported. Vaginal swabs obtained from 567 pregnant women at term or during labor were incubated for eight hrs in Todd Hewitt broth containing 15 micrograms per ml nalidixic acid, one microgram per ml polymixin, and 0.1 microgram per ml crystal violet (NPC broth). After streaking the swabs on blood...
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Detection of group B streptococcus (GBS) antigen in urine by latex particle agglutination (LPA) may facilitate the rapid diagnosis of GBS sepsis. We sought to compare three commercial LPA assays with specimens that were spiked with type-specific antigen, group-specific antigen, or type III organisms. There were sensitivity differences between the assays, but the Bactigen assay performed best, d...
متن کاملEvaluation of a new selective enrichment broth for detection of group B streptococci in pregnant women.
Studies at two Brown Medical School-affiliated hospitals were undertaken to evaluate a new selective broth medium (GBS broth) and to compare it to the LIM broth currently used to culture for group B streptococci. Beta-hemolytic group B streptococci produce a carotenoid pigment that turns GBS broth an orange color. From a total of 580 pregnant women, duplicate vaginal-rectal swabs were collected...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2001
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.39.1.408-409.2001